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KMID : 0363320040250010081
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Journal of Korean Oriental Internal Medicine
2004 Volume.25 No. 1 p.81 ~ p.91
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The Effects of Chungganhaeju-Tang on glutathione synthesis in HepG2 cell
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Yoon Yeo-Kwang
Kim Young-Chul
Lee Jang-Hoon
Woo Hong-Jung
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Abstract
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Objectives : The aim of this study is to investigate the inhibitory effect of Chungganhaeju-Tang on alcohol induced human hepatic cell apoptosis by synthesis of glutathione.
Methods : The amount of glutathione in HepG2 cell was measured with colorimetric glutathione assay kit and glutathione-conjugated CDNB(1-chloro-2,4-dinitrobenzene) at 37{circ}C and then measured by spectrometry to assess the activity of glutathione S-transferase.
Results : The synthesis of glutathione and the activity of glutathione S-transferase in HepG2 cell were promoted by Chungganhaeju-Tang and increased in dose/time-dependent manner. Chungganhaeju-Tang inhibited apoptosis induced by ethanol and acetaldehyde dependent to treatment dosage. In Buthione sulfoximine, a glutathione synthesis inhibitor, treated case, the synthesis of glutathione was inhibited and in Chungganhaeju-Tang treated case, the synthesis of glutathione is promoted with or without Buthione sulfoximine. The present findings suggest that Chungganhaeju-Tang inhibits alcohol induced apoptosis by synthesis of glutathione in HepG2 cell.
Conclusions : The result indicates that Chungganhaeju-Tang protects human hepatic cell by glutathione synthesis and made the liver recover from alcohol induced damage.
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KEYWORD
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Chungganhaeju-Tang, Glutathione, Apoptosis
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